Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles

We explored the coupling of laccases to magnetic nanoparticles (MNPs) with different surface chemical coating. Two laccase variants offering two opposite and precise orientations substrate oxidation site were immobilised onto core-shell MNPs presenting either aliphatic aldehyde, aromatic aldehyde or azide functional groups at particles surface. Oxidation capabilities six-resulting laccase-MNP hybrids compared on ABTS coniferyl alcohol. Herein, we show that original interfaces created differ substantially in their reactivities an amplitude from 1 > 4 folds depending nature substrate. Taking enzyme orientation into account design modification represents a way introduce selectivity catalysed reactions.